Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi.
Identifieur interne : 00A970 ( Main/Exploration ); précédent : 00A969; suivant : 00A971Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi.
Auteurs : J. Cox-Singh [Malaisie] ; A S Pomrehn ; H A Rahman ; R. Zakaria ; A O Miller ; B. SinghSource :
- International journal for parasitology [ 0020-7519 ] ; 1999.
Descripteurs français
- KwdFr :
- ADN des helminthes (isolement et purification), Animaux, Brugia malayi (génétique), Brugia malayi (isolement et purification), Faux négatifs, Filariose lymphatique (parasitologie), Filariose lymphatique (sang), Filariose lymphatique (épidémiologie), Humains, Malaisie (épidémiologie), Réaction de polymérisation en chaîne (), Sensibilité et spécificité.
- MESH :
- génétique : Brugia malayi.
- isolement et purification : ADN des helminthes, Brugia malayi.
- parasitologie : Filariose lymphatique.
- sang : Filariose lymphatique.
- épidémiologie : Filariose lymphatique, Malaisie.
- Animaux, Faux négatifs, Humains, Réaction de polymérisation en chaîne, Sensibilité et spécificité.
- Wicri :
- geographic : Malaisie.
English descriptors
- KwdEn :
- Animals, Brugia malayi (genetics), Brugia malayi (isolation & purification), DNA, Helminth (isolation & purification), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (parasitology), False Negative Reactions, Humans, Malaysia (epidemiology), Polymerase Chain Reaction (methods), Sensitivity and Specificity.
- MESH :
- chemical , isolation & purification : DNA, Helminth.
- geographic , epidemiology : Malaysia.
- blood : Elephantiasis, Filarial.
- epidemiology : Elephantiasis, Filarial.
- genetics : Brugia malayi.
- isolation & purification : Brugia malayi.
- methods : Polymerase Chain Reaction.
- parasitology : Elephantiasis, Filarial.
- Animals, False Negative Reactions, Humans, Sensitivity and Specificity.
Abstract
In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative.
PubMed: 10404266
Affiliations:
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Le document en format XML
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Cox-Singh</name><affiliation wicri:level="1"><nlm:affiliation>School of Medical Sciences, University of Science Malaysia, Kelantan, Malaysia. cox@tm.net.my</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>School of Medical Sciences, University of Science Malaysia, Kelantan</wicri:regionArea><wicri:noRegion>Kelantan</wicri:noRegion></affiliation></author><author><name sortKey="Pomrehn, A S" sort="Pomrehn, A S" uniqKey="Pomrehn A" first="A S" last="Pomrehn">A S Pomrehn</name></author><author><name sortKey="Rahman, H A" sort="Rahman, H A" uniqKey="Rahman H" first="H A" last="Rahman">H A Rahman</name></author><author><name sortKey="Zakaria, R" sort="Zakaria, R" uniqKey="Zakaria R" first="R" last="Zakaria">R. Zakaria</name></author><author><name sortKey="Miller, A O" sort="Miller, A O" uniqKey="Miller A" first="A O" last="Miller">A O Miller</name></author><author><name sortKey="Singh, B" sort="Singh, B" uniqKey="Singh B" first="B" last="Singh">B. 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Cox-Singh</name><affiliation wicri:level="1"><nlm:affiliation>School of Medical Sciences, University of Science Malaysia, Kelantan, Malaysia. cox@tm.net.my</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>School of Medical Sciences, University of Science Malaysia, Kelantan</wicri:regionArea><wicri:noRegion>Kelantan</wicri:noRegion></affiliation></author><author><name sortKey="Pomrehn, A S" sort="Pomrehn, A S" uniqKey="Pomrehn A" first="A S" last="Pomrehn">A S Pomrehn</name></author><author><name sortKey="Rahman, H A" sort="Rahman, H A" uniqKey="Rahman H" first="H A" last="Rahman">H A Rahman</name></author><author><name sortKey="Zakaria, R" sort="Zakaria, R" uniqKey="Zakaria R" first="R" last="Zakaria">R. Zakaria</name></author><author><name sortKey="Miller, A O" sort="Miller, A O" uniqKey="Miller A" first="A O" last="Miller">A O Miller</name></author><author><name sortKey="Singh, B" sort="Singh, B" uniqKey="Singh B" first="B" last="Singh">B. Singh</name></author></analytic><series><title level="j">International journal for parasitology</title><idno type="ISSN">0020-7519</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (isolation & purification)</term><term>DNA, Helminth (isolation & purification)</term><term>Elephantiasis, Filarial (blood)</term><term>Elephantiasis, Filarial (epidemiology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>False Negative Reactions</term><term>Humans</term><term>Malaysia (epidemiology)</term><term>Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (isolement et purification)</term><term>Animaux</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (isolement et purification)</term><term>Faux négatifs</term><term>Filariose lymphatique (parasitologie)</term><term>Filariose lymphatique (sang)</term><term>Filariose lymphatique (épidémiologie)</term><term>Humains</term><term>Malaisie (épidémiologie)</term><term>Réaction de polymérisation en chaîne ()</term><term>Sensibilité et spécificité</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Helminth</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Malaysia</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN des helminthes</term><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Malaisie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>False Negative Reactions</term><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Faux négatifs</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Malaisie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative.</div></front></TEI><affiliations><list><country><li>Malaisie</li></country></list><tree><noCountry><name sortKey="Miller, A O" sort="Miller, A O" uniqKey="Miller A" first="A O" last="Miller">A O Miller</name><name sortKey="Pomrehn, A S" sort="Pomrehn, A S" uniqKey="Pomrehn A" first="A S" last="Pomrehn">A S Pomrehn</name><name sortKey="Rahman, H A" sort="Rahman, H A" uniqKey="Rahman H" first="H A" last="Rahman">H A Rahman</name><name sortKey="Singh, B" sort="Singh, B" uniqKey="Singh B" first="B" last="Singh">B. Singh</name><name sortKey="Zakaria, R" sort="Zakaria, R" uniqKey="Zakaria R" first="R" last="Zakaria">R. Zakaria</name></noCountry><country name="Malaisie"><noRegion><name sortKey="Cox Singh, J" sort="Cox Singh, J" uniqKey="Cox Singh J" first="J" last="Cox-Singh">J. Cox-Singh</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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